Bristow R, Byrne J, Squirell J, Trencher H, Carter T, Rodgers B, Saman E, Duncan J
Murex Biotech Ltd., Dartford, Kent, UK.
J Acquir Immune Defic Syndr Hum Retrovirol. 1999 Apr 1;20(4):334-6. doi: 10.1097/00042560-199904010-00002.
The ability of cyclophilin to bind a panel of recombinant HIV-gag proteins was assessed using sensitive, quantitative, sandwich enzyme-linked immunosorbant assays (ELISAs). Significantly higher binding to cyclophilin was observed when recombinants contained at least 12 carboxy-terminal amino acids of p17 in addition to p24 sequences. These results indicate that the carboxy-terminus of p17 is important for optimal binding of cyclophilin to p24 and support the theory that cyclophilin acts on the uncleaved HIV-1 gag (p17-p24) precursor.
使用灵敏、定量的夹心酶联免疫吸附测定（ELISA）评估亲环蛋白与一组重组HIV - gag蛋白的结合能力。当重组体除了含有p24序列外还包含至少12个p17的羧基末端氨基酸时，观察到与亲环蛋白的结合显著更高。这些结果表明，p17的羧基末端对于亲环蛋白与p24的最佳结合很重要，并支持亲环蛋白作用于未切割的HIV - 1 gag（p17 - p24）前体的理论。
