Lai M, Skanchy D, Stobaugh J, Topp E
Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence 66047-2504, USA.
J Pharm Biomed Anal. 1998 Nov;18(3):421-7. doi: 10.1016/s0731-7085(98)00049-1.
A capillary electrophoresis (CE) method was developed for the separation of a model hexapeptide (L-Val-L-Tyr-L-Pro-L-Asn-Gly-L-Ala) and its degradation products. Separations using CE had much shorter analysis times than the RP-HPLC assay used previously. CE was also evaluated for possible use in analyzing peptide samples containing polymers. With a thorough rinsing procedure between runs, the presence of a model polymer did not affect the separation of the peptides. The CE assay can be applied to the study of peptide stability in polymers.
开发了一种毛细管电泳（CE）方法，用于分离一种模型六肽（L-缬氨酸-L-酪氨酸-L-脯氨酸-L-天冬酰胺-L-甘氨酸-L-丙氨酸）及其降解产物。与之前使用的反相高效液相色谱（RP-HPLC）分析相比，CE分离的分析时间要短得多。还评估了CE在分析含聚合物的肽样品中的可能用途。通过在运行之间进行彻底的冲洗程序，模型聚合物的存在不会影响肽的分离。CE分析可应用于聚合物中肽稳定性的研究。
