Prokazova N V, Zvezdina N D, Suslova I V, Korotaeva A A, Turpaev T M
Cardiology Research Centre, Moscow, Russia.
Ross Fiziol Zh Im I M Sechenova. 1998 Oct;84(10):969-78.
Chemical nature of the blood serum factor exerting cholinolytic effect on the animal heart, was determined. The effect is due to the serum lipid component: lysophosphatidylcholine. The latter in concentration 1-25 microM suppresses the sensitivity of the frog perfused heart ventricle to acetylcholine. Phosphatidylcholine was found to protect the heart from the lysophosphatidylcholine effect. Lysophosphatidylcholine also affected the binding of the acetylcholine high affinity antagonist [3H]-quinuclidinylbenzilate to the rabbit atria cell membranes. The mechanism of this effect is related to an increased ability of muscarinic receptors to form oligomeric complexes in presence of lysophosphatidylcholine, the latter manifesting a positive cooperativity on binding to ligand.
已确定对动物心脏发挥胆碱溶解作用的血清因子的化学性质。该作用归因于血清脂质成分：溶血磷脂酰胆碱。后者浓度为1 - 25微摩尔时可抑制蛙类离体灌流心室对乙酰胆碱的敏感性。发现磷脂酰胆碱可保护心脏免受溶血磷脂酰胆碱的影响。溶血磷脂酰胆碱还影响乙酰胆碱高亲和力拮抗剂[3H] - 喹核醇基苯甲酸酯与兔心房细胞膜的结合。这种作用机制与在溶血磷脂酰胆碱存在下毒蕈碱受体形成寡聚复合物的能力增强有关，溶血磷脂酰胆碱在与配体结合时表现出正协同性。
