非洲肺鱼原鳍鱼皮肤中阿黑皮素原（POMC）mRNA及POMC衍生肽的免疫定位

Proopiomelanocortin (POMC) mRNA and POMC-derived peptides immunolocalization in the skin of Protopterus annectens, an African lungfish.

作者信息

Masini M A, Sturla M, Pestarino M, Facchinetti F, Gallinelli A, Uva B M

机构信息

Department of Experimental, Environmental and Applied Biology, University of Genova, Italy.

出版信息

Peptides. 1999;20(1):87-91. doi: 10.1016/s0196-9781(98)00145-4.

DOI:10.1016/s0196-9781(98)00145-4

PMID:10098628

Abstract

Antisera against adrenocorticotropic hormone (ACTH), alpha-melanocyte stimulating hormone (alpha-MSH) and beta-endorphin were used to localize, by immunohistochemistry, proopiomelanocortin (POMC)-derived peptides in the skin excised from different regions of the African lungfish Protopterus annectens. Immunoreactivity was observed in the epidermis mainly in the germinal layer. Using human POMC cDNA as hybridization probe, POMC-like mRNA was identified in situ in epidermal cells. The demonstration in the same cells of POMC mRNA and POMC-related peptides immunoreactivity indicates a local production of opiate hormones.

摘要

利用抗促肾上腺皮质激素（ACTH）、α-黑素细胞刺激素（α-MSH）和β-内啡肽的抗血清，通过免疫组织化学方法，在非洲肺鱼原鳍鱼不同部位切除的皮肤中定位阿片促黑激素原（POMC）衍生肽。免疫反应主要在生发层的表皮中观察到。使用人POMC cDNA作为杂交探针，在表皮细胞中原位鉴定出POMC样mRNA。POMC mRNA和POMC相关肽免疫反应性在同一细胞中的证实表明阿片类激素是局部产生的。