On S L, Atabay H I, Corry J E
Danish Veterinary Laboratory, Copenhagen V, Denmark.
Epidemiol Infect. 1999 Feb;122(1):175-82. doi: 10.1017/s0950268898001824.
Eighteen strains of Campylobacter sputorum bv. paraureolyticus (isolated over a 12-month period from seven dairy cows contained in a single herd) were examined by resistotyping, and macrorestriction profiling using pulsed field gel electrophoresis (PFGE). The resistotypes of these strains were identical, although repeat testing indicated resistance to metronidazole was not a reliable trait for typing purposes. Five SmaI-derived genotypes were identified among the 18 strains. In 5 of 7 cows, isolates obtained from the same animal, but from different time periods, were genotypically indistinguishable, indicating persistence of infection. Macrorestriction profiles of 5 strains representing the 5 SmaI genotypes and 8 other strains of C. sputorum from various sources, were prepared using 4 endonucleases (SmaI, SalI, BamHI and KpnI). The only other strain of C. sputorum bv. paraureolyticus examined (a Canadian isolate from human faeces), was found to have a SmaI macrorestriction profile identical with one of the five clones isolated from the cattle. Moreover, SalI and BamHI profiles of all bv. paraureolyticus strains were similar, while digestion with KpnI was not observed. By contrast, the seven strains of C. sputorum bv. sputorum yielded various macrorestriction profiles with all the enzymes used, and features distinguishing the two biovars studied could be identified. This study indicates that C. sputorum can persist in cattle for at least 12 months and exhibits a clonal population genetic structure.
对18株解脲亚种弯曲杆菌（在12个月期间从一个牛群中的7头奶牛分离得到）进行了耐药谱分析，并使用脉冲场凝胶电泳（PFGE）进行了宏观限制性酶切图谱分析。这些菌株的耐药谱相同，不过重复检测表明甲硝唑耐药性并非用于分型的可靠特征。在这18株菌株中鉴定出了5种SmaI衍生基因型。在7头奶牛中的5头里，从同一头牛不同时间段分离得到的菌株在基因型上无法区分，表明感染具有持续性。使用4种核酸内切酶（SmaI、SalI、BamHI和KpnI）制备了代表5种SmaI基因型的5株菌株以及来自不同来源的8株其他解脲亚种弯曲杆菌菌株的宏观限制性酶切图谱。所检测的另一株解脲亚种弯曲杆菌（一株来自人类粪便的加拿大分离株），其SmaI宏观限制性酶切图谱与从牛群中分离得到的5个克隆之一相同。此外，所有解脲亚种菌株的SalI和BamHI图谱相似，而未观察到KpnI酶切。相比之下，7株解脲亚种弯曲杆菌对所用的所有酶均产生了不同的宏观限制性酶切图谱，并且可以鉴定出区分所研究的两个生物变种的特征。本研究表明解脲亚种弯曲杆菌可在牛体内持续存在至少12个月，并呈现出克隆群体遗传结构。
