Larsen-Su S, Krueger S K, Yueh M F, Lee M Y, Shehin S E, Hines R N, Williams D E
Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis 97331-7301, USA.
J Biochem Mol Toxicol. 1999;13(3-4):187-93. doi: 10.1002/(sici)1099-0461(1999)13:3/4<187::aid-jbt9>3.0.co;2-6.
Mammalian flavin-containing monooxygenase functions in the oxygenation of numerous xenobiotics containing a soft nucleophile, usually a nitrogen or sulfur. A total of five distinct flavin monooxygenase (FMO) isoforms are expressed in mammals. Individual isoforms are expressed in a sex-, age-, and tissue-specific fashion. In this study, we document the early developmental appearance of the major isoform in rabbit lung, FMO2. FMO2 catalytic activity as well as protein and mRNA are not only present in fetal and neonatal lung but, in some instances, approach levels found in the adult. The expression pattern of FMO2 is similar to that of the two major constitutive cytochromes P450 found in rabbit lung, 2B4 and 4B1. The early developmental appearance of these monooxygenases indicate an important role in the protection of the fetus and neonate against toxic insult from foreign chemicals.
哺乳动物含黄素单加氧酶参与众多含有软亲核试剂（通常为氮或硫）的外源化合物的氧化反应。哺乳动物共表达五种不同的黄素单加氧酶（FMO）同工型。个体同工型以性别、年龄和组织特异性方式表达。在本研究中，我们记录了兔肺中主要同工型FMO2在早期发育阶段的出现情况。FMO2的催化活性以及蛋白质和mRNA不仅存在于胎儿和新生儿肺中，而且在某些情况下接近成年动物中的水平。FMO2的表达模式与兔肺中发现的两种主要组成型细胞色素P450，即2B4和4B1的表达模式相似。这些单加氧酶在早期发育阶段的出现表明它们在保护胎儿和新生儿免受外来化学物质的毒性侵害方面具有重要作用。
