González G, Castro B, Massaldi H
Cátedra de Inmunología, Facultad de Química, Montevideo, Uruguay.
Biotechnol Bioeng. 1998 Jan 5;57(1):22-5.
Equine chorionic gonadotrophin (eCG) is a hormone of practical value in veterinary medicine and animal production. Here we report a novel preparation procedure based on its direct adsorption onto anionic-exchange resins in a batch-wise mode. The active plasma is previously conditioned to reduce pH and ionic strength to required levels. After the adsorption stage, a 90% recovery of the initial eCG is achieved, with a concentration factor of about 50 and an enrichment factor around 500, with high preservation of biological activity. Further purification is carried out by cation-exchange column chromatography. The recovery for the whole process is higher than 70%, and the final potency of the preparation is close to 4000 IU/mg. The process is well suited for its application to the industrial scale.
马绒毛膜促性腺激素（eCG）是一种在兽医学和动物生产中具有实用价值的激素。在此，我们报告一种基于将其以分批方式直接吸附到阴离子交换树脂上的新型制备方法。活性血浆预先进行预处理，将pH值和离子强度降低到所需水平。吸附阶段后，初始eCG的回收率达到90%，浓缩因子约为50，富集因子约为500，且生物活性得到高度保留。进一步通过阳离子交换柱色谱法进行纯化。整个过程的回收率高于70%，制剂的最终效价接近4000 IU/mg。该方法非常适合应用于工业规模。
