Kim E, Shin DH, Irwin DC, Wilson DB
Department of Biological Engineering, Inha University, Inchon, Korea.
Biotechnol Bioeng. 1998 Oct 5;60(1):70-6. doi: 10.1002/(sici)1097-0290(19981005)60:1<70::aid-bit8>3.0.co;2-y.
The factors affecting the production of a Thermomonospora fusca endoglucanase by a recombinant Streptomyces lividans strain were studied in a fermentor with glucose addition controlled by a pH-stat. The recombinant plasmid was stable for 35 generations with constant endoglucanase productivity. Glucose and peptone were used as the carbon and nitrogen sources. Addition of Tween-80 increased endoglucanase production twofold. A significant decrease in endoglucanase production was observed at low aeration. During fed-batch cultivation, pulse feeding (6 g/L) of a glucose-ammonium sulfate solution was optimal for endoglucanase production. With higher concentrations of glucose (15 g/L), a significant amount of organic acid, including acetic acid, was produced, which inhibited cell growth and endoglucanase production. Under optimum conditions, 1.7 U/mL of endoglucanase were produced. Copyright 1998 John Wiley & Sons, Inc.
在一个通过pH自动控制仪控制葡萄糖添加量的发酵罐中，研究了影响重组变铅青链霉菌菌株产生栖热放线菌内切葡聚糖酶的因素。重组质粒在35代中保持稳定，内切葡聚糖酶生产率恒定。葡萄糖和蛋白胨用作碳源和氮源。添加吐温80可使内切葡聚糖酶产量提高两倍。在低通气条件下，观察到内切葡聚糖酶产量显著下降。在补料分批培养过程中，脉冲添加（6 g/L）葡萄糖-硫酸铵溶液最有利于内切葡聚糖酶的产生。当葡萄糖浓度较高（15 g/L）时，会产生大量包括乙酸在内的有机酸，这抑制了细胞生长和内切葡聚糖酶的产生。在最佳条件下，可产生1.7 U/mL的内切葡聚糖酶。版权所有1998约翰威立父子公司。
