Sakai Y, Oishi A, Takahashi F
Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University, 2753 Ishii-machi, Utsunomiya 321-8585, Japan.
Biotechnol Bioeng. 1999 Feb 5;62(3):363-7.
An immobilized polyacrylamide gel containing beta-D-galactosidase and Sr-Ba-ferrite was magnetized in a static magnetic field. The gel rods (10 mm long, O 2 mm) exhibiting magnetic anisotropy could move at lower than 100 Hz but not at higher than 250 Hz in an alternating magnetic field of 200 Oe. In case of immovability of gel rods, the apparent enzymic activity increased 3 times higher under exposure of an alternating magnetic field of 500 Oe (570 Hz). It could be explained that the ferromagnetic powder inside the gel might vibrate under the influence of elasticity of gel in the alternating magnetic field of 100 or 500 Oe and 0.2-12 kHz. This might facilitate faster diffusion of the substance inside the gel and transportation of the substrate and the product through the surface of gel. Consequently, the enzyme reaction was apparently activated.
含有β-D-半乳糖苷酶和锶钡铁氧体的固定化聚丙烯酰胺凝胶在静磁场中被磁化。表现出磁各向异性的凝胶棒（10毫米长，直径2毫米）在200奥斯特的交变磁场中，在低于100赫兹时可以移动，但在高于250赫兹时则不能移动。在凝胶棒无法移动的情况下，在500奥斯特（570赫兹）的交变磁场作用下，表观酶活性增加了3倍。可以解释为，凝胶内部的铁磁粉末在100或500奥斯特以及0.2 - 12千赫兹的交变磁场中，可能会在凝胶弹性的影响下振动。这可能有助于凝胶内部物质更快地扩散，以及底物和产物通过凝胶表面的运输。因此，酶反应明显被激活。
