Demuth B, Jördening H J, Buchholz K
Lehrstuhl für Technologie der Kohlenhydrate, Technical University of Braunschweig, Langer Kamp 5, D-38106 Braunschweig, Germany.
Biotechnol Bioeng. 1999 Mar 5;62(5):583-92. doi: 10.1002/(sici)1097-0290(19990305)62:5<583::aid-bit10>3.0.co;2-4.
Dextransucrase catalyses the formation of dextran, but also of numerous oligosaccharides from sucrose and different acceptors, if appropriate conditions are chosen. Much experimental work has been carried out and a scheme of reactions and a mathematical model have been developed to describe the complex kinetic behaviour of the enzyme. A computer program was used to calculate the parameters of the model from a broad range of experimental data, investigating a large number of kinetic tests with the acceptors maltose and fructose. The results lead to design considerations for a continuous reactor system with immobilized dextransucrase to produce leucrose, a disaccharide of industrial interest.
葡聚糖蔗糖酶催化葡聚糖的形成，但在选择合适条件时，也能催化蔗糖与不同受体形成多种低聚糖。已经开展了大量实验工作，并建立了反应方案和数学模型来描述该酶复杂的动力学行为。使用计算机程序从广泛的实验数据中计算模型参数，研究了大量以麦芽糖和果糖为受体的动力学测试。结果为设计一个固定化葡聚糖蔗糖酶的连续反应器系统以生产工业上感兴趣的二糖异麦芽酮糖醇提供了设计思路。
