Buchman C A, Luxford W M, Hirsch B E, Fucci M J, Kelly R H
Department of Otolaryngology, University of Miami School of Medicine, Florida, USA.
Am J Otol. 1999 Mar;20(2):174-8.
Western blot assay for beta-2 transferrin protein is a clinically useful method for the detection of human perilymph and should be used for the diagnosis of perilymph fistulas (PLFs).
Considerable controversy exists regarding the diagnosis of PLF. Recent studies suggest that the detection of beta-2 transferrin protein may be useful in the identification of perilymph.
To evaluate the usefulness of the beta-2 transferrin assay for identifying human perilymph, paired perilymph samples and negative controls were collected on Gelfoam pledgets from 20 patients who had surgery that opened the inner ear. Blinded immunoelectrophoretic assay (Western blot) for beta-2 transferrin was performed on each specimen.
Only one (5%) of the known perilymph samples and none of the control specimens were definitely positive for beta-2 transferrin. Combined with historical data, this assay has 29% sensitivity, 100% specificity, 100% positive predictive value, and 31% negative predictive value.
These findings suggest that the beta-2 transferrin protein assay may not be a reliable method for detecting human perilymph when performed using this technique.
β-2转铁蛋白的蛋白质免疫印迹法是检测人外淋巴液的一种临床有用方法，应用于外淋巴瘘（PLF）的诊断。
关于PLF的诊断存在相当大的争议。近期研究表明，β-2转铁蛋白的检测可能有助于识别外淋巴液。
为评估β-2转铁蛋白检测法识别出人外淋巴液的有效性，从20例接受内耳开放手术的患者中，在明胶海绵上收集配对的外淋巴液样本和阴性对照。对每个样本进行β-2转铁蛋白的盲法免疫电泳检测（免疫印迹法）。
已知的外淋巴液样本中仅1份（5%）β-2转铁蛋白呈明确阳性，对照样本均为阴性。结合既往数据，该检测法的灵敏度为29%，特异度为100%，阳性预测值为100%，阴性预测值为31%。
这些结果表明，使用该技术进行β-2转铁蛋白检测时，可能不是检测人外淋巴液的可靠方法。
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