Bordure P, Delaroche O, Beauvillain C, Legent F
Service d'ORL, Hôtel-Dieu, CHR de Nantes.
Ann Otolaryngol Chir Cervicofac. 1994;111(4):180-4.
beta 2-transferrin is a specific protein found in the cerebrospinal fluid and in the perilymph. Detecting beta 2-transferrin in the middle ear is of great interest when a fistula of the perilymph is suspected. This protein can be detected on microsamples with an immunofixation technique. We searched for beta 2-transferrin in pure perilymph, cerebrospinal fluid and serum in 8 patients operated by translabyrinthine approach for acoustic tumor removal. Search for beta 2-transferrin was performed in liquid from the inner ear in 3 labyrinthectomies. Samples were taken on collagen sponges or with micro syringes. beta 2-transferrin was detected in the perilymph of patients operated for neurinoma and in 2 of the 3 labyrinthectomies. This protein was found in only one of the patients for which the diagnosis of perilymphatic fistula had been retained. Detection of beta 2-transferrin in the middle ear can be proposed as a specific diagnostic test for perilymphatic fistula when the clinical situation does not suggest a fistula involving cerebrospinal fluid.
β2-转铁蛋白是一种存在于脑脊液和外淋巴中的特殊蛋白质。当怀疑存在外淋巴瘘时，检测中耳中的β2-转铁蛋白具有重要意义。这种蛋白质可以通过免疫固定技术在微量样本中检测到。我们在8例接受经迷路入路手术切除听神经瘤的患者的纯外淋巴、脑脊液和血清中寻找β2-转铁蛋白。在3例迷路切除术患者的内耳液体中进行了β2-转铁蛋白的检测。样本通过胶原海绵或微量注射器采集。在接受神经鞘瘤手术患者的外淋巴以及3例迷路切除术中的2例中检测到了β2-转铁蛋白。在仅保留外淋巴瘘诊断的患者中，仅在1例患者中发现了这种蛋白质。当临床情况不提示涉及脑脊液的瘘时，中耳β2-转铁蛋白的检测可作为外淋巴瘘的特异性诊断试验。
