Schumacher G, Kataoka M, Roth J A, Mukhopadhyay T
Department of General Surgery and Transplantation, Charite Campus Virchow Klinikum, Berlin, Germany.
Clin Cancer Res. 1999 Mar;5(3):493-9.
We examined the effect of 2-methoxyestradiol (2-ME) on the growth and tumorigenesis of human pancreatic cancer cells. 2-ME inhibited the growth of these cell lines (50-90%) in a dose- and time-dependent fashion, and terminal deoxynucleotidyl transferase staining showed that it induced apoptotic cell death. Flow cytometric analysis indicated that 2-ME-sensitive cells showed a prolonged S phase after 48 h of treatment. We used a mouse model for in vivo studies of lung metastasis and injected MIA PaCa-2 cells into the tail veins of nu/nu mice; lung colonies were formed. Mice given oral 2-ME showed 60% inhibition in the number of lung colonies compared with control, untreated animals. These results suggest that 2-ME may have clinical application for the treatment of pancreatic cancer.
我们研究了2-甲氧基雌二醇（2-ME）对人胰腺癌细胞生长和肿瘤发生的影响。2-ME以剂量和时间依赖性方式抑制这些细胞系的生长（50%-90%），末端脱氧核苷酸转移酶染色显示它诱导凋亡性细胞死亡。流式细胞术分析表明，对2-ME敏感的细胞在处理48小时后显示S期延长。我们使用小鼠模型进行肺转移的体内研究，并将MIA PaCa-2细胞注入裸鼠尾静脉；形成了肺集落。与未处理的对照动物相比，口服2-ME的小鼠肺集落数量抑制了60%。这些结果表明，2-ME可能在胰腺癌治疗中具有临床应用价值。
