Yoshida A, Nakano Y, Yamashita Y, Oho T, Shibata Y, Ohishi M, Koga T
First Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Kyushu University, Fukuoka, Japan.
FEBS Lett. 1999 Mar 12;446(2-3):287-91. doi: 10.1016/s0014-5793(99)00237-9.
The nucleotide sequence of the dnaK operon cloned from Porphyromonas gingivalis revealed that the operon does not contain homologues of either dnaJ or grpE. However, there were two genes which encode small heat shock proteins immediately downstream from the dnaK and they were transcribed together with dnaK as one unit. The ATPase activity of the P. gingivalis DnaK was synergistically stimulated up to 40-fold in the simultaneous presence of Escherichia coli DnaJ and GrpE. These results suggest that the DnaK homologue of P. gingivalis, with its unique genetic structure and evolutionary features, works as a member of the DnaK chaperone system.
从牙龈卟啉单胞菌克隆的dnaK操纵子的核苷酸序列显示，该操纵子不包含dnaJ或grpE的同源物。然而，在dnaK下游紧邻有两个编码小热休克蛋白的基因，它们与dnaK一起作为一个单元转录。在同时存在大肠杆菌DnaJ和GrpE的情况下，牙龈卟啉单胞菌DnaK的ATP酶活性协同增强了40倍。这些结果表明，牙龈卟啉单胞菌的DnaK同源物具有独特的遗传结构和进化特征，作为DnaK伴侣系统的一员发挥作用。
