甘蓝（Brassica oleracea var. capitata）磷脂酶D的分子克隆与功能表达
Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata).
作者信息
Kim D U, Roh T Y, Lee J, Noh J Y, Jang Y J, Hoe K L, Yoo H S, Choi M U
机构信息
Department of Chemistry and Center for Molecular Catalysis, Seoul National University, Seoul 151-742, South Korea.
出版信息
Biochim Biophys Acta. 1999 Mar 25;1437(3):409-14. doi: 10.1016/s1388-1981(99)00020-7.
We cloned and expressed a full-length cDNA encoding a phospholipase D of type alpha (PLDalpha) from cabbage. Analysis of the cDNA predicted an 812-amino-acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDalpha and castor bean PLD, respectively. Expression of this cDNA clone in E. coli shows a functional PLD activity similar to that of the natural PLD.
我们从卷心菜中克隆并表达了一个编码α型磷脂酶D（PLDα）的全长cDNA。对该cDNA的分析预测其编码一个由812个氨基酸组成、分子量为92.0 kDa的蛋白质。推导得到的卷心菜PLD氨基酸序列与拟南芥PLDα和蓖麻PLD的一致性分别为83%和80%。该cDNA克隆在大肠杆菌中的表达显示出与天然PLD相似的功能性PLD活性。
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