Hoeffel D P, Hinrichs S H, Garvin K L
Department of Orthopaedic Surgery and Rehabilitation, University of Nebraska Medical Center, Omaha 68198, USA.
Clin Orthop Relat Res. 1999 Mar(360):37-46. doi: 10.1097/00003086-199903000-00007.
The last decade has seen the crossover of basic molecular research techniques toward clinical application. The use of molecular diagnostics originally was driven by the desire to identify unculturable or fastidious pathogens. Success in this setting has led to new applications aimed at identifying organisms involved in musculoskeletal infection. Recently, polymerase chain reaction techniques have been investigated as to their efficacy in diagnosing septic arthritis and periprosthetic infection. This paper will offer an introduction to molecular diagnostics, a brief review of several techniques, and update their status in the diagnosis of musculoskeletal infection.
在过去十年中，基础分子研究技术已向临床应用领域交叉渗透。分子诊断技术的应用最初是受识别不可培养或苛求病原菌的需求所驱动。在这方面取得的成功催生了旨在识别参与肌肉骨骼感染的病原体的新应用。最近，人们对聚合酶链反应技术在诊断化脓性关节炎和假体周围感染方面的功效进行了研究。本文将介绍分子诊断技术，简要回顾几种技术，并更新它们在肌肉骨骼感染诊断中的现状。
