Lewin-Kowalik J, Górka D, Larysz-Brysz M, Goka B, Swiech-Sabuda E, Małecka-Tendera E, Krause M
Department of Physiology, Silesian Medical University, Katowice, Poland.
Acta Physiol Hung. 1997;85(3):259-68.
Our previous studies revealed that predegenerated peripheral nerve grafts facilitated neurite outgrowth from the injured hippocampus and that this effect was particularly distinct when 7-, 28-, and 35-days predegenerated nerve grafts were used. It is recently known that a totally transected peripheral nerve exhibits biphasic neurite-promoting activity. The early phase lasts 7 days. The aim of the present study was to find whether short-time predegenerated (1-6 days) peripheral nerve grafts exert any neurotrophic effect and when this influence is maximal. Experiments were carried out on adult male Wistar rats. Sciatic nerves were totally transected and following 1, 2, 3, 4, 5 and 6 days their distal stumps were implanted into the hippocampus. Control animals were treated with non-predegenerated sciatic nerve grafts. In all groups FITC-HRP was injected into the free end of graft six weeks following surgery. Special histochemic technique showed AChE-positive fibres inside the grafts of all examined groups. Fluorescence microscopic examination revealed the labeled cells in all examined groups, however their number was different in each group, depending on the predegeneration stage. They were most numerous at the fourth day of predegeneration.
我们之前的研究表明，预变性的周围神经移植物能促进损伤海马体的神经突生长，且当使用预变性7天、28天和35天的神经移植物时，这种效果尤为明显。最近已知，完全横断的周围神经具有双相促神经突活性。早期持续7天。本研究的目的是探究短期预变性（1 - 6天）的周围神经移植物是否具有任何神经营养作用，以及这种影响何时最大。实验在成年雄性Wistar大鼠身上进行。坐骨神经被完全横断，在1、2、3、4、5和6天后，将其远端残端植入海马体。对照动物接受未预变性的坐骨神经移植物治疗。术后六周，在所有组中，将异硫氰酸荧光素 - 辣根过氧化物酶（FITC - HRP）注入移植物的游离端。特殊组织化学技术显示，所有检查组的移植物内均有乙酰胆碱酯酶（AChE）阳性纤维。荧光显微镜检查在所有检查组中均发现了标记细胞，然而，每组中细胞数量不同，这取决于预变性阶段。在预变性第4天时细胞数量最多。
