Eppig J J, Eckhardt R A
Differentiation. 1976 Aug 3;6(2):97-103. doi: 10.1111/j.1432-0436.1976.tb01475.x.
Soluble and microsomal proteins synthesized at various stages of oogenesis in Xenopus laevis were compared by 3H and 14C dual-isotope labelling with subsequent mixing and analysis on sodium dodecyl sulfate (SDS)-polyacrylamide gels. The results indicated that the proteins labelled in all the stages of oogenesis studied are remarkably similar. However, the patterns in the small and medium-sized oocytes are more similar to one another than to the patterns characteristic of large oocytes. The greatest differences were found when comparing the microsomal proteins. The labelling patterns of oocyte proteins in vitro were not significantly different from the in vivo patterns for the stages of oogenesis studied. These results indicate that (1) there is little quantitative contribution of proteins to either the soluble or microsomal fractions from extra-oocytic sources in vivo and (2) the in vitro system itself has little effect on the labelling patterns over the incubation period.
通过3H和14C双同位素标记，随后在十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶上进行混合和分析，比较了非洲爪蟾卵子发生不同阶段合成的可溶性蛋白和微粒体蛋白。结果表明，在所研究的卵子发生的所有阶段标记的蛋白质非常相似。然而，中小型卵母细胞中的模式彼此之间比与大型卵母细胞特有的模式更相似。在比较微粒体蛋白时发现了最大的差异。在所研究的卵子发生阶段，卵母细胞蛋白的体外标记模式与体内模式没有显著差异。这些结果表明：（1）体内来自卵母细胞外来源的蛋白质对可溶性或微粒体部分的定量贡献很小；（2）体外系统本身在孵育期对标记模式影响很小。
