Bhat M A, Izaddoost S, Lu Y, Cho K O, Choi K W, Bellen H J
Howard Hughes Medical Institute, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
Cell. 1999 Mar 19;96(6):833-45. doi: 10.1016/s0092-8674(00)80593-0.
Polarization of epithelial cells depends on a hierarchical process whereby specific membrane-associated proteins become targeted to specialized membrane domains. Here, we describe a novel Drosophila protein, Discs Lost (DLT), that plays a crucial role in the polarization of embryonic epithelia during cellular blastoderm formation. At subsequent stages of development, DLT interacts with the apical determinant Crumbs (CRB) and the laterally localized protein Neurexin IV (NRX IV). Mutations in dlt or double-stranded RNA interference lead to aberrant localization of CRB and NRX IV and cause a concomitant loss of epithelial cell polarity. Hence, DLT is required to establish and maintain cell polarity and participates in different molecular complexes that define apical and lateral membrane domains.
上皮细胞的极化取决于一个分级过程，在此过程中，特定的膜相关蛋白靶向到特殊的膜结构域。在这里，我们描述了一种新的果蝇蛋白，盘状缺失（DLT），它在细胞胚盘形成过程中胚胎上皮的极化中起关键作用。在随后的发育阶段，DLT与顶端决定因子Crb（CRB）和侧向定位蛋白神经纤毛蛋白IV（NRX IV）相互作用。dlt中的突变或双链RNA干扰导致CRB和NRX IV的异常定位，并导致上皮细胞极性的伴随丧失。因此，DLT是建立和维持细胞极性所必需的，并参与定义顶端和侧向膜结构域的不同分子复合物。
