Taki A, Nakatani Y, Misugi K, Yao M, Nagashima Y
Department of Pathology, Yokohama City University School of Medicine, Yokohama, Japan.
Mod Pathol. 1999 Mar;12(3):310-7.
Chromophobe renal cell carcinoma (RCC) is a newly established category of RCC composed histologically of characteristic "chromophobe" tumor cells. Although ultrastructural and immunohistochemical studies showed that these tumor cells present several features similar to those found in the intercalated cells of the collecting duct, immunohistochemical studies using antibody panels on a large number of cases are limited. We performed an immunohistochemical study of 21 Japanese cases of chromophobe RCC, along with cases of clear RCC and renal oncocytoma, to find hallmarks useful for precise differential diagnosis of these tumors. Chromophobe RCC was positive for epithelial membrane antigen but negative for vimentin. Cytokeratins did not show constant immunoreactivity in the three types of renal tumors. Furthermore, all of the chromophobe RCCs and renal oncocytomas were positive for E-cadherin but not for N-cadherin, whereas all of the clear RCCs were negative for E-cadherin, and 58% were positive for N-cadherin. The Ki-67 labeling indices were significantly lower in cases classified as (pT1) or Grade 2 chromophobe RCC than in cases of clear RCC. Immunoreaction for E-cadherin was demonstrated to be useful for distinguishing chromophobe RCC from clear RCC, and a low Ki-67 labeling index might indicate a favorable prognosis, as reported in several previous studies.
嫌色性肾细胞癌（RCC）是一种新确立的RCC类型，其组织学上由特征性的“嫌色”肿瘤细胞组成。尽管超微结构和免疫组织化学研究表明这些肿瘤细胞呈现出一些与集合管闰细胞中发现的特征相似的特征，但使用抗体组合对大量病例进行的免疫组织化学研究有限。我们对21例日本嫌色性RCC病例以及透明RCC和肾嗜酸细胞瘤病例进行了免疫组织化学研究，以寻找有助于对这些肿瘤进行精确鉴别诊断的标志物。嫌色性RCC上皮膜抗原呈阳性，波形蛋白呈阴性。细胞角蛋白在这三种类型的肾肿瘤中未表现出恒定的免疫反应性。此外，所有嫌色性RCC和肾嗜酸细胞瘤E-钙黏蛋白呈阳性，N-钙黏蛋白呈阴性，而所有透明RCC E-钙黏蛋白呈阴性，58% N-钙黏蛋白呈阳性。分类为（pT1）或2级的嫌色性RCC病例的Ki-67标记指数显著低于透明RCC病例。正如之前几项研究所报道的，E-钙黏蛋白的免疫反应被证明有助于将嫌色性RCC与透明RCC区分开来，低Ki-67标记指数可能表明预后良好。
