Ziegler R, Willingham L A, Engler D L, Tolman K J, Bellows D, Van Der Horst D J, Yepiz-Plascencia G M, Law J H
Institute of Zoology, Animal Physiology, Martin-Luther-Universität Halle-Wittenberg, Germany.
Eur J Biochem. 1999 Apr;261(1):285-90. doi: 10.1046/j.1432-1327.1999.00276.x.
A new type of insect lipoprotein was isolated from the hemolymph of the female cochineal insect Dactylopius confusus. The lipoprotein from the cochineal insect hemolymph was found to have a relative molecular mass of 450 000. It contains 48% lipid, mostly diacylglycerol, phospholipids and hydrocarbons. The protein moiety of the lipoprotein consists of two apoproteins of approximately 25 and 22 kDa, both of which are glycosylated. Both apolipoproteins are also found free in the hemolymph, unassociated with any lipid. Purified cochineal apolipoproteins can combine with Manduca sexta lipophorin, if injected together with adipokinetic hormone into M. sexta. This could indicate that the cochineal lipoprotein can function as a lipid shuttle similar to lipophorins of other insects, and that the cochineal insect apolipoproteins have an overall structure similar to insect apolipophorin-III.
从雌性胭脂虫（Dactylopius confusus）的血淋巴中分离出一种新型昆虫脂蛋白。发现来自胭脂虫血淋巴的脂蛋白相对分子质量为450000。它含有48%的脂质，主要是二酰甘油、磷脂和碳氢化合物。该脂蛋白的蛋白质部分由两种分子量约为25 kDa和22 kDa的载脂蛋白组成，两者均为糖基化。这两种载脂蛋白在血淋巴中也以游离形式存在，不与任何脂质结合。如果将纯化的胭脂虫载脂蛋白与促脂动激素一起注射到烟草天蛾（Manduca sexta）体内，它们可以与烟草天蛾的脂转运蛋白结合。这可能表明胭脂虫脂蛋白可以像其他昆虫的脂转运蛋白一样作为脂质穿梭载体发挥作用，并且胭脂虫的载脂蛋白具有与昆虫载脂蛋白III相似的整体结构。
