Rampon C, Peyron C, Gervasoni D, Pow D V, Luppi P H, Fort P
Neurobiologie des Etats de Sommeil et d'Eveil, INSERM U480, Lyon, France.
Eur J Neurosci. 1999 Mar;11(3):1058-66. doi: 10.1046/j.1460-9568.1999.00511.x.
The amino acid glycine is a major inhibitory neurotransmitter in the brainstem and is likely involved in the tonic inhibition of the monoaminergic neurons during all sleep-waking stages. In order to determine the neurons at the origin of the glycinergic innervation of the two principal monoaminergic nuclei, the locus coeruleus and the dorsal raphe of the rat, we applied a double-labelling technique, combining retrograde transport of cholera-toxin B subunit with glycine immunohistochemistry. Using this technique, we found that the locus coeruleus and dorsal raphe nuclei receive a common glycinergic innervation from the ventral and ventrolateral periaqueductal grey, including the adjacent deep mesencephalic reticular nucleus. Small additional glycinergic inputs to these nuclei originated from the lateral paragigantocellular nucleus and the rostral ventromedial medullary reticular formation. The potential role of these glycinergic inputs in the control of the excitability of the monoaminergic neurons of the locus coeruleus and dorsal raphe nuclei is discussed.
氨基酸甘氨酸是脑干中的一种主要抑制性神经递质，可能在所有睡眠-觉醒阶段对单胺能神经元的紧张性抑制中发挥作用。为了确定大鼠两个主要单胺能核团（蓝斑和中缝背核）甘氨酸能神经支配的起源神经元，我们应用了一种双重标记技术，将霍乱毒素B亚基的逆行转运与甘氨酸免疫组织化学相结合。使用该技术，我们发现蓝斑和中缝背核接受来自腹侧和腹外侧导水管周围灰质（包括相邻的中脑深部网状核）的共同甘氨酸能神经支配。这些核团的少量额外甘氨酸能输入来自外侧巨细胞旁核和延髓头端腹内侧网状结构。本文讨论了这些甘氨酸能输入在控制蓝斑和中缝背核单胺能神经元兴奋性方面的潜在作用。
