Grigsby J S, Bergen W G, Merkel R A
Growth. 1976 Dec;40(4):303-16.
The effect of testosterone administration on muscle development and protein synthesis was studied with intact male rabbits. Rabbits were randomized into three experimental groups; day 0 control (G-1), day 15 control (G-2) and day 15 testosterone (G-3). Testosterone (delta4-Androsten-17beta-ol-3-one in silastic tubing) was surgically implanted into the G-3 rabbits. On day (G-1) and day 15 (G-2 and 3) of the experiment all rabbits were injected IP with 50 mu Ci-3H-leucine. Rabbits were sacrificed at intervals after the 3H-leucine injection. Right and left semitendinosus muscles, pituitary and adrenal glands, testicles and blood samples were obtained for analysis. Testosterone improved (P less than .01) gain and feed efficiency, depressed testicle weight (P less than .01), increased serum testosterone and insulin (P less than 0.01) but did not affect adrenal and pituitary weights. Muscle moisture, fat and total protein, myofibrillar, sarcoplasmic and stromal protein fractions and RNA and DNA content were not significantly affected by testosterone although G-3 semitendinosus muscle had the highest total and myofibrillar protein, RNA, and DNA content. The testosterone treated rabbits (G-3) had higher (P less than 0.05) 3H-leucine incorporation into myofibrillar protein and lower 3H-leucine incorporation into sarcoplasmic protein than the day 0 and day 15 controls.
通过完整的雄性兔子研究了睾酮给药对肌肉发育和蛋白质合成的影响。兔子被随机分为三个实验组：第0天对照组（G-1）、第15天对照组（G-2）和第15天睾酮组（G-3）。将睾酮（硅橡胶管中的δ4-雄甾烯-17β-醇-3-酮）通过手术植入G-3组兔子体内。在实验的第0天（G-1）和第15天（G-2和G-3），所有兔子均经腹腔注射50μCi -3H-亮氨酸。在注射3H-亮氨酸后每隔一段时间处死兔子。获取左右半腱肌、垂体、肾上腺、睾丸和血样进行分析。睾酮改善了（P<0.01）增重和饲料效率，降低了睾丸重量（P<0.01），增加了血清睾酮和胰岛素（P<0.01），但不影响肾上腺和垂体重量。睾酮对肌肉水分、脂肪和总蛋白、肌原纤维、肌浆和基质蛋白组分以及RNA和DNA含量没有显著影响，尽管G-3组半腱肌的总蛋白和肌原纤维蛋白、RNA和DNA含量最高。与第0天和第15天的对照组相比，接受睾酮治疗的兔子（G-3）肌原纤维蛋白中3H-亮氨酸掺入率更高（P<0.05），而肌浆蛋白中3H-亮氨酸掺入率更低。
