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来自烟草花叶病毒感染植物的多聚核糖体相关RNA的体外翻译

In vitro translation of polyribosome-associated RNAs from tobamovirus-infected plants.

作者信息

Skotnicki A, Gibbs A, Shaw D C

出版信息

Intervirology. 1976;7(4-5):256-71. doi: 10.1159/000149958.

Abstract

RNAs associated with polyribosomes in plants infected with the U2 strain of tobacco mosaic virus (TMV) or with sunnhemp mosaic virus have been isolated. Most are about 0.35 X 10(6) daltons in weight. They translate efficiently in vitro to produce their respective coat proteins which were identified by their serological behavior and peptide composition. They also reassemble in vitro with coat protein. The coat protein of sunnhemp mosaic virus reassembles more quickly than that of TMV U2.

摘要

已分离出感染烟草花叶病毒(TMV)U2株或印度麻花叶病毒的植物中与多核糖体相关的RNA。大多数RNA的分子量约为0.35×10⁶道尔顿。它们在体外能高效翻译以产生各自的外壳蛋白,这些外壳蛋白通过血清学行为和肽组成得以鉴定。它们还能在体外与外壳蛋白重新组装。印度麻花叶病毒的外壳蛋白比TMV U2的外壳蛋白重新组装得更快。

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