Mortimer R G, Berndtson W E, Ennen B D, Pickett B W
J Dairy Sci. 1976 Dec;59(12):2134-7. doi: 10.3168/jds.S0022-0302(76)84499-2.
Semen from each of six bulls was used to study the effects of percent glycerol and rate of freezing on spermatozoa extended in egg yolk-sodium citrate extender and packaged in .25-ml Continental straws. Treatments were arranged factorially and included 5, 6, 7, 8, 9, 10, or 11% (volume/volume) glycerol; freezing rates (time from 5 to -130 C) of 1, 2, 3.5, 7, 12, or 20 min; and post-thaw incubation times of 0 and 2 h at 38 C. Post-thaw motility was similar for spermatozoa frozen in 2 to 20 min, and all means were greater than for spermatozoa frozen in 1 min. Post-thaw motility of spermatozoa frozen in 7% glycerol did not differ from that with 5 to 9% but was superior to that with 10 or 11% glycerol. Although glycerol and freezing rate interacted, 7% glycerol provided survival equal to or greater than that with the other percents of glycerol, regardless of freezing rate.
使用来自六头公牛的精液，研究甘油百分比和冷冻速率对在蛋黄 - 柠檬酸钠稀释液中稀释并包装在0.25毫升大陆型细管中的精子的影响。处理采用析因设计，包括5%、6%、7%、8%、9%、10%或11%（体积/体积）的甘油；冷冻速率（从5℃到 -130℃的时间）为1分钟、2分钟、3.5分钟、7分钟、12分钟或20分钟；以及解冻后在38℃下的孵育时间为0小时和2小时。在2至20分钟内冷冻的精子解冻后的活力相似，且所有平均值均高于在1分钟内冷冻的精子。在7%甘油中冷冻的精子解冻后的活力与在5%至9%甘油中冷冻的精子没有差异，但优于在10%或11%甘油中冷冻的精子。尽管甘油和冷冻速率存在相互作用，但无论冷冻速率如何，7%甘油提供的存活率等于或高于其他百分比的甘油。
