Sekhon A S, Padhye A A
Mycopathologia. 1976 Dec 10;60(1):33-7. doi: 10.1007/BF00442545.
Nineteen isolates of Ctenomyces serratus including three pairs of tester strains were crossed in all possible combinations on diluted Pablum cereal agar with added salts, oatmeal salts agar-Medium E, and soil and hair medium. Out of 190 crosses, 18 were fertile and the remaining 172 were sterile. Of the 19 isolates, seven were '-' mating type, 11 were '+' mating type, and one (UAMH 1959) was non-fertile with the other isolates. The high degree of non-fertile crosses among the 19 isolates shows that the use of mating reactions for the identification of conidial isolates of C. serratus may not be a practical tool, unless a large number of isolates belonging to both the mating types are employed. The production of exudate droplets in the fertile crosses seemed to be correlated with the degree of mating response. Ascocarp morphology corresponded closely to that of a cleistothecium, because of the completely closed nature. Ascospores were quite alike in their size, but they differed in shape, depending on their orientation.
19株锯齿梳霉分离株(包括3对测试菌株)在添加了盐的稀释婴儿米粉琼脂、燕麦盐琼脂 - E培养基以及土壤和毛发培养基上进行了所有可能组合的杂交。在190次杂交中,18次杂交可育,其余172次杂交不育。在这19株分离株中,7株为“-”交配型,11株为“+”交配型,还有1株(UAMH 1959)与其他分离株杂交不育。这19株分离株之间高度不育的杂交表明,除非使用大量属于两种交配型的分离株,否则利用交配反应来鉴定锯齿梳霉的分生孢子分离株可能不是一种实用的方法。在可育杂交中渗出液滴的产生似乎与交配反应的程度相关。子囊果形态与闭囊壳非常相似,因为其完全封闭的性质。子囊孢子大小相当,但形状不同,这取决于它们的取向。