Lüschow U, Schulz-Harder J
Biochim Biophys Acta. 1978 Sep 22;512(2):377-87. doi: 10.1016/0005-2736(78)90261-4.
We developed a new procedure which induces multifunctional reagents to crosslink at one interface between a black bilayer and the adjacent water phase. This procedure yields "bilayer-gel" membranes, i.e. membranes consisting of a bilayer and a polymer layer. The bilayer-gel membrane may tentatively be considered to be a new membrane system, because the formation of the polymer layer changes some bilayer properties. We studied bilayer-gel membranes composed of a bilayer of oxidized cholesterol and of a polymer layer of poly-L-lysine crosslinked by glutardialdehyde. Compared to unmodified bilayers, this membrane system has an electrical conductance of the same magnitude, the same electrical capacity and similar shapes of current-voltage dependences. However, this system is asymmetrical and differs in ion selectivity and increased stability from an unmodified bilayer.
我们开发了一种新方法，可促使多功能试剂在黑色双层膜与相邻水相之间的一个界面处发生交联。该方法可产生“双层-凝胶”膜，即由双层膜和聚合物层组成的膜。双层-凝胶膜可初步被视为一种新的膜系统，因为聚合物层的形成改变了一些双层膜的特性。我们研究了由氧化胆固醇双层膜和经戊二醛交联的聚-L-赖氨酸聚合物层组成的双层-凝胶膜。与未修饰的双层膜相比，该膜系统具有相同量级的电导、相同的电容以及相似的电流-电压依赖性形状。然而，该系统是不对称的，在离子选择性和稳定性方面与未修饰的双层膜有所不同，稳定性有所提高。
