Bogh L D, Duling T A
Department of Pathology, University of Iowa, College of Medicine, Iowa City 52242.
Clin Lab Sci. 1993 May-Jun;6(3):167-73.
To describe the basic components of a flow cytometer and the features unique to research and clinical instruments.
Recent review articles and textbooks on cytometry or laboratory diagnosis.
Not applicable.
Performed by the authors.
A cytometer is used for counting and measuring the physical and chemical characteristics of cells and other biological particles. A flow cytometer is different from other cytometers in that a single-cell suspension is passed through it in a fluid stream. A flow cytometer is a complex combination of optics, fluidics, and electronics. A labeled cell is forced through the system, causing the cell to scatter light and emit fluorescence. This output is sensed by photodetectors and then amplified and converted to digital signals for storage in computers. This stored data can be displayed or used for further analysis.
Some instruments can separate cells that meet certain preselected criteria. Therefore, the flow cytometer is widely used in research as well as in clinical immunology and hematology to perform rapid immunophenotyping, cell sorting, and DNA analysis.
描述流式细胞仪的基本组成部分以及研究型和临床型仪器所独有的特征。
近期关于细胞计数或实验室诊断的综述文章和教科书。
不适用。
由作者完成。
细胞仪用于计数和测量细胞及其他生物颗粒的物理和化学特性。流式细胞仪与其他细胞仪的不同之处在于，单细胞悬液以液流形式通过它。流式细胞仪是光学、流体学和电子学的复杂组合。标记的细胞被迫通过该系统，导致细胞散射光并发出荧光。此输出由光电探测器检测，然后放大并转换为数字信号以存储在计算机中。这些存储的数据可以显示或用于进一步分析。
一些仪器可以分离符合某些预先选定标准的细胞。因此，流式细胞仪在研究以及临床免疫学和血液学中被广泛用于进行快速免疫表型分析、细胞分选和DNA分析。
