Bilirubin in liquor amnii. A rapid and exact method for quantitative determination.

A method is described for an exact and rapid determination of bilirubin in the amniotic fluid. Errors due to turbidity and to contamination of hemoglobin or debris are elliminated because bilirubin is extracted directely into chloroform by a special technique and determined by direct spectrophotometry. The new technique is related to the well known Liley procedure and the results with the new method are plotted into a modified Liley-sceme.