Armbruster D A, Hawes L C, Winter C T
Armstrong Laboratory, Human Systems Center, Brooks Air Force Base, Texas 78235.
J Int Fed Clin Chem. 1992 Sep;4(4):166-73.
The accuracy and precision of the Packard Probe 1000, a computer-driven robotic sample processor, were determined using NCCLS Guideline 18-P, employing a rigorous gravimetric procedure. For volumes of 25, 100, and 200 muL, inaccuracy ranged from -0.5 to +0.6% (saline) and -0.8 to +0.7% (human serum), and imprecision from 0.1 to 0.6% (saline) and 0.02 to 0.8% (human serum), in the dip and sip mode of pipetting. For the same volumes of saline in the dispense-through pipetting mode, inaccuracy ranged from -0.1 to -0.9% and imprecision from 0.1 to 0.5%. To obtain this level of performance, the instrument's operating parameters were changed from the factory default settings. This automated liquid-handling system is capable of impressive accuracy and precision, but it must be calibrated in the same manner as any other volumetric device. Performance varies with the volume, type of liquid, and mode of pipetting. Inaccuracy of less than or equal to 1.0% and imprecision of less than =1.0% can be attained along with rapid, walkaway pipetting of calibrators, controls, and large numbers of patient samples.
使用NCCLS指南18 - P，采用严格的重量分析法，测定了计算机驱动的机器人样本处理器帕卡德探针1000的准确性和精密度。在移液的吸取和吐出模式下，对于25、100和200微升的体积，误差范围为 - 0.5%至 + 0.6%（生理盐水）和 - 0.8%至 + 0.7%（人血清），不精密度范围为0.1%至0.6%（生理盐水）和0.02%至0.8%（人血清）。在连续分液移液模式下，对于相同体积的生理盐水，误差范围为 - 0.1%至 - 0.9%，不精密度范围为0.1%至0.5%。为达到这种性能水平，仪器的操作参数已从出厂默认设置更改。这种自动液体处理系统具有令人印象深刻的准确性和精密度，但必须像任何其他容量装置一样进行校准。性能会因体积、液体类型和移液模式而有所不同。通过对校准物、对照品和大量患者样本进行快速、无需值守的移液操作，可实现误差小于或等于1.0%且不精密度小于等于1.0%。
