Schulze W, Wollenberger A
Acta Biol Med Ger. 1976;35(7):837-43.
An attempt was made to demonstrate adenylate cyclase (ATP pyrophosphate-lyase (cyclizing) EC 4.6.1.1) activity in white and red skeletal muscle (M. psoas and M. soleus) of guinea pigs cytochemically by the lead precipitation technique. 5'-Adenylylimidodiphosphate served as substrate. The free lead ion concentration in the incubation medium was kept below the value that is toxic to the enzyme. An electron dense reaction product, presumably lead imidodiphosphate, was deposited in the soleus muscle (red muscle fibers) at all parts of the plasma membrane and in the psoas muscle (white muscle fibers) chiefly at the membranes of the sarcoplasmic reticulum. The reaction at the latter site was enhanced by adrenaline; the enhancement was prevented by propranolol. These findings are in good agreement with results that have been obtained by other authors in experiments on subcellular fractions of the two types of muscle.
通过铅沉淀技术，试图以细胞化学方法证明豚鼠白色和红色骨骼肌（腰大肌和比目鱼肌）中腺苷酸环化酶（ATP 焦磷酸裂解酶（环化），EC 4.6.1.1）的活性。5'-腺苷酰亚胺二磷酸用作底物。孵育介质中的游离铅离子浓度保持在对该酶有毒的值以下。一种电子致密反应产物，推测为铅亚胺二磷酸，沉积在比目鱼肌（红色肌纤维）的质膜各处，而在腰大肌（白色肌纤维）中主要沉积在肌浆网的膜上。肾上腺素可增强后一部位的反应；普萘洛尔可阻止这种增强。这些发现与其他作者在对这两种肌肉的亚细胞部分进行实验时所获得的结果非常一致。
