Weeks O B, Francesconi M D
J Bacteriol. 1978 Nov;136(2):614-24. doi: 10.1128/jb.136.2.614-624.1978.
The neutral lipid fraction of the photochromogenic, coryneform bacterium Cellulomonas dehydrogenans (Arnaudi 1942) comb. nov. contains the sterol precursor squalene and at least two sterols, cholesterol and beta-sitosterol. The compounds were characterized by mass spectrometry and combination gas-liquid chromatography--mass spectrometry. De novo sterol biosynthetic ability was shown from incorporation of 14C from D-[U-14C]glucose into squalene and the sterol fraction. The squalene concentration approximated 0.002 to 0.005% of the total dry cell weight, and the sterols approximated 0.03 to 0.05%.
光致变色棒状细菌脱氢纤维单胞菌(Arnaudi,1942年)新组合的中性脂质部分含有甾醇前体角鲨烯以及至少两种甾醇,即胆固醇和β-谷甾醇。这些化合物通过质谱法以及气相色谱-质谱联用进行了表征。通过将D-[U-¹⁴C]葡萄糖中的¹⁴C掺入角鲨烯和甾醇部分,证明了其从头合成甾醇的能力。角鲨烯浓度约占细胞总干重的0.002%至0.005%,甾醇约占0.03%至0.05%。