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脱氢纤维单胞菌(阿尔瑙迪,1942年)中角鲨烯和甾醇的存在情况 新组合。赫斯特,1971年。

Occurrence of squalene and sterols in Cellulomonas dehydrogenans (Arnaudi 1942) comb. nov. Hester 1971.

作者信息

Weeks O B, Francesconi M D

出版信息

J Bacteriol. 1978 Nov;136(2):614-24. doi: 10.1128/jb.136.2.614-624.1978.

Abstract

The neutral lipid fraction of the photochromogenic, coryneform bacterium Cellulomonas dehydrogenans (Arnaudi 1942) comb. nov. contains the sterol precursor squalene and at least two sterols, cholesterol and beta-sitosterol. The compounds were characterized by mass spectrometry and combination gas-liquid chromatography--mass spectrometry. De novo sterol biosynthetic ability was shown from incorporation of 14C from D-[U-14C]glucose into squalene and the sterol fraction. The squalene concentration approximated 0.002 to 0.005% of the total dry cell weight, and the sterols approximated 0.03 to 0.05%.

摘要

光致变色棒状细菌脱氢纤维单胞菌(Arnaudi,1942年)新组合的中性脂质部分含有甾醇前体角鲨烯以及至少两种甾醇,即胆固醇和β-谷甾醇。这些化合物通过质谱法以及气相色谱-质谱联用进行了表征。通过将D-[U-¹⁴C]葡萄糖中的¹⁴C掺入角鲨烯和甾醇部分,证明了其从头合成甾醇的能力。角鲨烯浓度约占细胞总干重的0.002%至0.005%,甾醇约占0.03%至0.05%。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7861/218586/761fc4afe833/jbacter00288-0169-a.jpg

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