Haggag H F, Ganguli N C
J Dairy Sci. 1978 Oct;61(10):1384-91. doi: 10.3168/jds.S0022-0302(78)83739-4.
Incorporation of amino acids by mitochondria and its subfractions from mammary gland of the goat during lactation was studied in vitro. Assessment of distribution of radioactivity incorporated by intact mitochondria into its subfractions revealed maximum specific activity in the inner membrane. During lactation the inner membrane of mitochondria exhibited further stimulation in such incorporation. Matrix was the next active fraction whereas outer membrane and peripheral space had negligible radioactivity. In isolated submitochondrial fractions from mammary gland incubated with radioactive amino acids under similar conditions of assay, the inner membrane was the most potent subfraction in incorporation. In mammary tissue during lactation this fraction was the most active site. The relative sequence of the subfractions appeared to remain unaltered in lactation and was in the order of inner membrane greater than matrix greater than outer membrane greater than peripheral space. The ratio of specific activity in inner to outer membrane appears to increase significantly during lactation.
研究了泌乳期山羊乳腺线粒体及其亚组分对氨基酸的体外掺入情况。对完整线粒体掺入的放射性在其亚组分中的分布评估显示，内膜的比活性最高。泌乳期间，线粒体的内膜在这种掺入中表现出进一步的刺激作用。基质是下一个活性组分，而外膜和外周间隙的放射性可忽略不计。在类似测定条件下，用放射性氨基酸孵育的乳腺分离亚线粒体组分中，内膜是掺入能力最强的亚组分。在泌乳期的乳腺组织中，该组分是最活跃的部位。亚组分的相对顺序在泌乳期似乎保持不变，顺序为内膜＞基质＞外膜＞外周间隙。泌乳期间，内膜与外膜的比活性之比似乎显著增加。
