一种使用Ficoll-Hypaque梯度培养PHA诱导的小鼠淋巴细胞的改良方法。
An improved method for PHA-culturing mouse lymphocytes using Ficoll-Hypaque gradient.
作者信息
Giraudo Conesa L C, Rumi L, Pasqualini C D
出版信息
Boll Ist Sieroter Milan. 1976 Sep 30;55(4):304-7.
PMID:1016583
Abstract
A simple, reproducible method is described for in vitro culture of mouse lymphocytes purified by differential centrifugation through Ficoll-Hypaque gradient. Peripheral blood, spleen and thymus purified lymphocytes responded well to in vitro PHA stimulation as measured by blast transformation and tritiated thymidine incorporation; the lowest values were observed with thymus lymphocytes.
摘要
描述了一种简单、可重复的方法，用于通过Ficoll-Hypaque梯度差速离心纯化的小鼠淋巴细胞的体外培养。通过胚细胞转化和氚化胸腺嘧啶核苷掺入法测定，外周血、脾脏和胸腺纯化淋巴细胞对体外PHA刺激反应良好；胸腺淋巴细胞的数值最低。
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