[家兔纤维蛋白原的制备及免疫比浊定量分析]
[Preparation and immunoephelometric quantitation of fibrinogen in rabbits].
作者信息
Dhermy D, Judon C, Engler R, Jayle M F
出版信息
Biochimie. 1976;58(11-12):1311-20.
PMID:1016652
Abstract
Pure rabbit fibrinogen was prepared by a method involving two ammonium sulfate precipitations, one 2 M phosphate buffer precipitation, one DEAE cellulose chromatography and lastly one Sepharose 6 B chromatography. The aminoacid composition was determined and an immunonephelemetric assay was proposed. This assay followed an accurate determination of fibrinogen concentration in a rabbit with inflammatory reaction.
摘要
纯兔纤维蛋白原是通过一种包含两次硫酸铵沉淀、一次2M磷酸缓冲液沉淀、一次DEAE纤维素层析以及最后一次琼脂糖6B层析的方法制备的。测定了其氨基酸组成并提出了一种免疫比浊法。该方法是在准确测定一只患有炎症反应的兔子体内纤维蛋白原浓度之后进行的。
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