Szmigielski S, Jeljaszewicz J
Cancer Lett. 1976 May;1(5):299-303. doi: 10.1016/s0304-3835(75)98042-8.
Functional tests of granulopoiesis (leukocytosis, bone marrow picture, incubation in vitro of bone marrow with [3H]thymidine followed by radioautography and counting of labeled promyelocytes and myelocytes, serum muramidase level, liberation of granulocyte bone marrow reserve, Nitro-BT reduction in blood granulocytes, enzyme cytochemistry, and phagocytosis) were performed in rabbits given bubulphan (10 mg/kg) or 5-fluorouracil (200 mg/kg). Determinations were carried on serially during treatment with cytostatics. Some of the cytostatic-treated animals received intravenous (i.v.) injections of purified staphylococcal leukocidin in daily doses of 0.1 mg/kg. In control animals, theleukocidin resulted in stimulation of granulopoiesis (leukocytosis, increased number of [3H]thymidine-labeled myelocytes, elevated serum muramidase level). Animals receiving cytostatics suffered from marked inhibition of granulopoiesis accompanied by decrease of bone marrow granulocyte reserve. Injection of staphylococcal leukocidin during the period of myelosuppression evoked by cytostatics resulted in partial protection of granylopoiesis and faster regeneration of the leukocyte system.
对给予布布芬（10毫克/千克）或5-氟尿嘧啶（200毫克/千克）的兔子进行了粒细胞生成功能测试（白细胞增多、骨髓图像、用[3H]胸腺嘧啶核苷体外培养骨髓，随后进行放射自显影并计数标记的早幼粒细胞和中幼粒细胞、血清溶菌酶水平、粒细胞骨髓储备的释放、血液粒细胞中硝基蓝四氮唑还原、酶细胞化学和吞噬作用）。在使用细胞抑制剂治疗期间连续进行测定。一些接受细胞抑制剂治疗的动物每天静脉注射0.1毫克/千克纯化的葡萄球菌杀白细胞素。在对照动物中，杀白细胞素导致粒细胞生成受到刺激（白细胞增多、[3H]胸腺嘧啶核苷标记的中幼粒细胞数量增加、血清溶菌酶水平升高）。接受细胞抑制剂的动物粒细胞生成受到明显抑制，同时骨髓粒细胞储备减少。在细胞抑制剂引起骨髓抑制期间注射葡萄球菌杀白细胞素导致粒细胞生成部分得到保护，白细胞系统再生加快。
