来自大鼠肝细胞核的多胺依赖性脱氧核糖核酸酶活性

Polyamine-dependent deoxyribonuclease activity from rat-liver nuclei.

作者信息

Nakano T, Tsuboi M

出版信息

J Biochem. 1976 Dec;80(6):1435-8. doi: 10.1093/oxfordjournals.jbchem.a131417.

DOI:10.1093/oxfordjournals.jbchem.a131417

Abstract

When nuclei isolated from rat liver in a low salt buffer were washed with 0.1 M NaCl solution, the supernatant showed a deoxyribonuclease (DNase) activity. The activity required Mg2+ and in addition spermine or spermidine, and its optimal pH was 7.2-7.4. The activity was higher on denatured (single stranded) DNA than on double-helical DNA. With both substrates the activity was highest at a polyamine concentration at which the DNA-polyamine complex began to precipitate. No Mg2++Ca2+ dependent DNase activity was detected in the preparation.

摘要

当用低盐缓冲液从大鼠肝脏中分离出的细胞核用0.1M NaCl溶液洗涤时，上清液显示出脱氧核糖核酸酶（DNase）活性。该活性需要Mg2+，此外还需要精胺或亚精胺，其最适pH为7.2 - 7.4。该活性对变性（单链）DNA的作用比对双链DNA的作用更强。对于两种底物，在DNA - 多胺复合物开始沉淀的多胺浓度下活性最高。在该制剂中未检测到Mg2+ + Ca2+依赖性DNase活性。