多胺诱导的DNA聚集。
Polyamine induced aggregation of DNA.
作者信息
Osland A, Kleppe K
出版信息
Nucleic Acids Res. 1977 Mar;4(3):685-95. doi: 10.1093/nar/4.3.685.
Abstract
Polyamine induced aggregation of various DNAs has been studied under conditions usually employed in many enzymatic assays where DNA is one of the substrates. Spermine was by far the most efficient polyamine in causing aggregation followed by spermidine and cadaverine. All double-stranded and naturally occurring single-stranded DNAs were found to aggregate. No aggregation of single-stranded homodeoxypolymers could be detected under the same conditions. The concentration of polyamine at which the aggregation commenced was found to be a linear function of the DNA concentration. The slope of the curves depended on the nature of the polyamine, DNA the concentration of Mg++ and the ionic strength.
摘要
在许多以DNA为底物之一的酶促测定中通常采用的条件下，研究了多胺诱导的各种DNA聚集情况。到目前为止，精胺是引起聚集最有效的多胺，其次是亚精胺和尸胺。发现所有双链和天然存在的单链DNA都会聚集。在相同条件下未检测到单链同型脱氧聚合物的聚集。发现聚集开始时的多胺浓度是DNA浓度的线性函数。曲线的斜率取决于多胺性质、DNA、Mg++浓度和离子强度。
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