[中间芽孢杆菌和短小芽孢杆菌分泌型鸟苷特异性核糖核酸酶基因在枯草芽孢杆菌细胞中的表达]
[Expression of secreted guanyl-specific ribonuclease genes from Bacillus intermedius and Bacillus pumilus in Bacillus subtilis cells].
作者信息
Znamenskaia L V, Vershinina O A, Vershinina V I, Krasnov S I, Kostrov S V, Akimkina T V, Leshchinskaia I B, Hartley R W
出版信息
Mol Gen Mikrobiol Virusol. 1999(1):12-7.
Plasmids with whole genes for ribonucleases from B. intermedius (binase) and B. pumilis (RNase Bp) assembled with the whole gene of barstar, a specific intracellular inhibitor, are constructed. The resultant plasmids pMZ55 and pMZ56 effectively express binase and RNase Bp genes in B. subtilis cells. A medium for maximum expression of RNase genes by recombinant strains is developed. The expression of binase and RNase Bp genes in B. subtilis cells is negatively regulated by exogenic inorganic phosphate.
构建了含有来自中间芽孢杆菌（binase）和短小芽孢杆菌（RNase Bp）的核糖核酸酶全基因，并与特异性细胞内抑制剂barstar的全基因组装在一起的质粒。所得质粒pMZ55和pMZ56在枯草芽孢杆菌细胞中有效表达binase和RNase Bp基因。开发了一种用于重组菌株最大程度表达核糖核酸酶基因的培养基。枯草芽孢杆菌细胞中binase和RNase Bp基因的表达受到外源无机磷酸盐的负调控。
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