[利用聚合酶链反应检测猪霍乱病毒]
[Detection of the hog cholera virus using the polymerase chain reaction].
作者信息
Semenikhin V I, Puzyrev A T, Oreshkova S F, Donchenko A S, Chekishev V M, Il'ichev A A
出版信息
Mol Gen Mikrobiol Virusol. 1999(1):27-30.
PMID:10190108
Abstract
A rapid and highly sensitive method for detecting hog cholera virus (HCV) based on a reverse transcription of the polymerase chain reaction (RT-PCR) is developed. Primers complementary to the most homologous sites of virus genome in an area coding the precursor for glycoproteins gp44/gp48 are selected. Detection of the virus in pathological material by the RT-PCR showed that use of these primers in amplification allows detection of different HCV strains.
摘要
开发了一种基于聚合酶链反应逆转录（RT-PCR）的快速且高度灵敏的检测猪霍乱病毒（HCV）的方法。选择了与病毒基因组中编码糖蛋白gp44/gp48前体的区域中最同源位点互补的引物。通过RT-PCR对病理材料中的病毒进行检测表明，在扩增中使用这些引物能够检测不同的HCV毒株。
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