[Polymorphism of HLA-DRB1, DQB1 in the Hans of north China].

OBJECTIVE

To understand polymorphism of HLA-DRB1, DQB1 in the Hans of north China and obtain more comprehensive and accurate data on genetics at DNA level.

METHODS

Polymerase chain reaction-sequence specific primers (PCR-SSP) were used to determine HLA-DRB1, DQB1 alleles in 107 unrelated healthy Han individuals of north China.

RESULTS

The authors determined 14 DRB1, 9 DQB1 alleles, which included not only the allele frequencies that corresponded to the gene frequencies of DR, DQ loci determined by other 9 cooperating labs but also the allele frequencies of DRB115, DRB116, DQB10301, DQB10302, DQB10303, DQB105, DQB10601, DQB10602, and DQB1*0604 that other serology labs did not report.

CONCLUSION

This study has obtained a more comprehensive and accurate data set of the normal allele frequencies and linkage disequilibrium parameters of HLA-DRB1, DQB1 in the Hans of north China, which may be of significance in the studies on population genetics and disease association.