Li W, Sun Y, Shan X, Zhi H, Yuan Q, Ge J, Zhang Z
HLA Laboratory, Beijing Red Cross Blood Center, Beijing, 100088, P. R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 1999 Apr;16(2):74-6.
To understand polymorphism of HLA-DRB1, DQB1 in the Hans of north China and obtain more comprehensive and accurate data on genetics at DNA level.
Polymerase chain reaction-sequence specific primers (PCR-SSP) were used to determine HLA-DRB1, DQB1 alleles in 107 unrelated healthy Han individuals of north China.
The authors determined 14 DRB1, 9 DQB1 alleles, which included not only the allele frequencies that corresponded to the gene frequencies of DR, DQ loci determined by other 9 cooperating labs but also the allele frequencies of DRB115, DRB116, DQB10301, DQB10302, DQB10303, DQB105, DQB10601, DQB10602, and DQB1*0604 that other serology labs did not report.
This study has obtained a more comprehensive and accurate data set of the normal allele frequencies and linkage disequilibrium parameters of HLA-DRB1, DQB1 in the Hans of north China, which may be of significance in the studies on population genetics and disease association.
了解中国北方汉族人群中HLA-DRB1、DQB1基因多态性，在DNA水平上获取更全面、准确的遗传学数据。
采用聚合酶链反应-序列特异性引物（PCR-SSP）技术，对107名中国北方汉族无血缘关系的健康个体进行HLA-DRB1、DQB1等位基因分型。
共检出14个DRB1等位基因和9个DQB1等位基因，其中不仅包含与其他9个协作实验室所检测的DR、DQ基因座基因频率相对应的等位基因频率，还包含其他血清学实验室未报道的DRB115、DRB116、DQB10301、DQB10302、DQB10303、DQB105、DQB10601、DQB10602和DQB1*0604等位基因频率。
本研究获得了中国北方汉族人群中HLA-DRB1、DQB1正常等位基因频率及连锁不平衡参数的较为全面、准确的数据集，这对群体遗传学及疾病关联研究可能具有重要意义。
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