使用聚乙烯亚胺（PEI）/DNA/腺病毒系统对乳腺上皮细胞进行瞬时转染。

Transient transfection of mammary epithelial cells with a PEI/DNA/adenovirus system.

作者信息

Bischof J, Vietor I, Cotten M, Huber L A

机构信息

Institute of Molecular Pathology, Vienna, Austria.

出版信息

Biol Chem. 1999 Feb;380(2):269-73. doi: 10.1515/BC.1999.036.

DOI:10.1515/BC.1999.036

PMID:10195435

Abstract

Studies on epithelial cells often require the transient expression of exogenous proteins in polarized epithelial cells. However, the major limitation of this approach has been the difficulty of obtaining transient gene expression in polarized epithelial cell cultures. We report here on the application of a polyethylenimine (PEI)/DNA/adenovirus system for efficient transient gene expression in mammary epithelial cells. Based on luciferase assay and FACScan analysis the PEI/DNA/adenovirus system is shown to be an effective and simple method for transfecting epithelial cells.

摘要

对上皮细胞的研究常常需要在极化上皮细胞中瞬时表达外源蛋白。然而，这种方法的主要局限在于难以在极化上皮细胞培养物中实现瞬时基因表达。我们在此报告一种聚乙烯亚胺（PEI）/DNA/腺病毒系统在乳腺上皮细胞中高效瞬时基因表达的应用。基于荧光素酶检测和流式细胞仪分析，PEI/DNA/腺病毒系统被证明是一种转染上皮细胞的有效且简便的方法。