Francisco-Ortega J, Fuertes-Aguilar J, Gómez-Campo C, Santos-Guerra A, Jansen R K
Department of Biological Sciences and Fairchild Tropical Garden, Florida International University, University Park, Miami, FL, 33199, USA.
Mol Phylogenet Evol. 1999 Apr;11(3):361-80. doi: 10.1006/mpev.1998.0592.
Crambe L. (Brassicaceae) is an Old World genus with a disjunct distribution among four major centers of species diversity. A phylogenetic analysis of nucleotide sequences of the internal transcribed spacers (ITS) of the nuclear ribosomal repeat was conducted with 27 species of Crambe and 18 related genera. Cladistic analyses using weighted and unweighted parsimony support Crambe as a monophyletic genus with three major lineages. The first comprises those taxa endemic to the Macaronesian archipelagos. Taxa with a predominant Mediterranean distribution form the second assemblage, and a disjunction between east Africa (C. abyssinica) and the Mediterranean (C. hispanica) occurs in this clade. The third lineage includes all Eurosiberian-Asian taxa and C. kilimandscharica, a species from the highlands of east Africa. A basal biogeographic split between east Africa and Eurasia is present in the third clade. The patterns of relationships in the ITS tree are concordant with known climatic events in northern Africa and southwestern Asia since the middle Miocene. The ITS trees are congruent with the current sectional classification except for a few members of sections Crambe, Leptocrambe, and Orientecrambe (C. cordifolia, C. endentula, C. kilimandscharica, and C. kotschyana). Low levels of support in the basal branches do not allow resolution of which genera of the subtribes Raphaniae or Brassicinae are sister to Crambe. Both subtribes appear to be highly polyphyletic in the ITS trees.
海甘蓝属（十字花科）是一个旧世界属，在四个主要物种多样性中心呈间断分布。对核糖体重复序列内部转录间隔区（ITS）的核苷酸序列进行了系统发育分析，研究对象包括27种海甘蓝属植物和18个相关属。使用加权和非加权简约法的分支分析支持海甘蓝属为单系属，有三个主要谱系。第一个谱系包括那些特有的马卡罗尼西亚群岛的分类群。主要分布在地中海地区的分类群构成了第二个组合，在这个分支中，东非（阿比西尼亚海甘蓝）和地中海地区（西班牙海甘蓝）之间存在间断分布。第三个谱系包括所有欧洲-西伯利亚-亚洲的分类群以及来自东非高地的物种基利曼沙尔海甘蓝。在第三个分支中存在东非和欧亚大陆之间的基础生物地理分歧。ITS树中的亲缘关系模式与自中新世中期以来北非和西亚已知的气候事件一致。除了海甘蓝属、细叶海甘蓝属和东方海甘蓝属的一些成员（心叶海甘蓝、无齿海甘蓝、基利曼沙尔海甘蓝和科氏海甘蓝）外，ITS树与当前的组分类一致。基部支系的支持度较低，无法确定萝卜亚族或芸苔亚族的哪些属是海甘蓝属的姊妹属。在ITS树中，这两个亚族似乎都是高度多系的。
