Tang K L, Berendzen P B, Wiley E O, Morrissey J F, Winterbottom R, Johnson G D
Department of Systematics and Ecology, University of Kansas, Lawrence, Kansas, 66045, USA.
Mol Phylogenet Evol. 1999 Apr;11(3):415-25. doi: 10.1006/mpev.1998.0596.
Fragments of 12S and 16S mitochondrial DNA genes were sequenced for 14 acanthuroid taxa (representing all six families) and seven outgroup taxa. The combined data set contained 1399 bp after removal of all ambiguously aligned positions. Examination of site saturation indicated that loop regions of both genes are saturated for transitions, which led to a weighted parsimony analysis of the data set. The resulting tree topology generally agreed with previous morphological hypotheses, most notably placing the Luvaridae within the Acanthuroidei, but it also differed in several areas. The putative sister group of Acanthuroidei, Drepane, was recovered within the suborder, and the sister group of the family Acanthuridae, Zanclus, was likewise recovered within the family. Morphological characters were included to produce a combined data set of 1585 characters for 14 acanthuroid taxa and a single outgroup taxon. An analysis of the same 15 taxa was performed with only the DNA data for comparison. The total-evidence analysis supports the monophyly of the Acanthuridae. A parametric bootstrap suggests the possibility that the paraphyly of Acanthuridae indicated by the molecular analyses is the result of long-branch attraction. The disagreement between molecular and morphological data on the relationships of the basal acanthuroids and its putative sister taxon is unresolved.
对14种刺尾鱼目分类单元（代表所有六个科）和7个外类群分类单元的12S和16S线粒体DNA基因片段进行了测序。去除所有比对不明确的位置后，合并数据集包含1399个碱基对。对位点饱和度的检查表明，两个基因的环区在转换方面已饱和，这导致对该数据集进行加权简约分析。所得的树形拓扑结构总体上与先前的形态学假设一致，最显著的是将副刺尾鱼科置于刺尾鱼亚目内，但在几个方面也存在差异。刺尾鱼亚目的假定姐妹群——副棘鲆，在该亚目内被发现，而刺尾鱼科的姐妹群——镰鱼，同样在该科内被发现。纳入形态学特征以产生一个包含14个刺尾鱼目分类单元和一个单一外类群分类单元的1585个特征的合并数据集。对相同的15个分类单元仅使用DNA数据进行分析以作比较。全证据分析支持刺尾鱼科的单系性。参数自展分析表明，分子分析所显示的刺尾鱼科并系性可能是长枝吸引的结果。关于基部刺尾鱼及其假定姐妹分类单元之间关系的分子数据与形态学数据之间的分歧尚未得到解决。
