Nielsen R, Palsbøll P J
Department of Integrative Biology, University of California, Berkeley, California, 94720, USA.
Mol Phylogenet Evol. 1999 Apr;11(3):477-84. doi: 10.1006/mpev.1998.0597.
We evaluate some common simulation procedures as well as a recently developed likelihood method used for testing hypotheses regarding microsatellite evolution. Results from simulated data revealed that the tests for the detection of multi-step mutations in general have some power, whereas tests for the presence of constraints on the repeat number have only very limited power. The tests were applied to population data obtained from nine different baleen whale populations. High agreement was found between results obtained using the simulation-based approach and results obtained using a likelihood ratio test. In four of the nine population samples the tests rejected the one-step mutation model. In two instances the significant deviation was due to excess of heterozygosity and in two instances to a reduced level of heterozygosity relative to the expectations under the stepwise mutation model. The former significant deviation was consistent with occasional multi-step mutations, whereas the latter may indicate the presence of constraints on the number of repeats.
我们评估了一些常见的模拟程序以及一种最近开发的用于检验关于微卫星进化假设的似然方法。模拟数据的结果表明，一般来说，检测多步突变的测试具有一定效力，而检测重复次数是否存在限制的测试效力非常有限。这些测试应用于从九个不同须鲸种群获得的群体数据。使用基于模拟的方法获得的结果与使用似然比检验获得的结果之间发现高度一致。在九个群体样本中的四个样本中，测试拒绝了一步突变模型。在两个实例中，显著偏差是由于杂合度过高，而在两个实例中是由于相对于逐步突变模型下的预期杂合度水平降低。前一种显著偏差与偶尔的多步突变一致，而后者可能表明存在对重复次数的限制。
