Debby-Brafman A, Burstyn-Cohen T, Klar A, Kalcheim C
Department of Anatomy and Cell Biology, Hebrew University, Hadassah Medical School, Jerusalem 91120, Israel.
Neuron. 1999 Mar;22(3):475-88. doi: 10.1016/s0896-6273(00)80703-5.
Neural crest (NC) cells migrate exclusively into the rostral half of each sclerotome, where they avoid the dermomyotome and the paranotochordal sclerotome. F-spondin is expressed in these inhibitory regions and throughout the caudal halves. In vitro bioassays of NC spreading on substrates of rostral or caudal epithelial-half somites (RS or CS, respectively) revealed that NC cells adopt on RS a fibroblastic morphology, whereas on CS they fail to flatten. F-spondin inhibited flattening of NC cells on RS. Conversely, F-spondin antibodies prevented rounding up of NC cells on CS. Addition of F-spondin to trunk explants inhibited NC migration into the sclerotome, and treatment of embryos with anti-F-spondin antibodies yielded migration into otherwise inhibitory sites. Thus, somite-derived F-spondin is an inhibitory signal involved in patterning the segmental migration of NC cells and their topographical segregation within the RS.
神经嵴（NC）细胞仅迁移至每个体节的前半部分，在此它们避开了生皮节和脊索旁体节。F-腱蛋白在这些抑制区域以及整个后半部分均有表达。对NC细胞在体节前半部分或后半部分上皮（分别为RS或CS）底物上的铺展进行的体外生物测定显示，NC细胞在RS上呈现成纤维细胞形态，而在CS上则无法扁平铺展。F-腱蛋白抑制了NC细胞在RS上的扁平铺展。相反，F-腱蛋白抗体可防止NC细胞在CS上变圆。向躯干外植体中添加F-腱蛋白会抑制NC细胞向体节的迁移，而用抗F-腱蛋白抗体处理胚胎则会使NC细胞迁移至原本具有抑制作用的部位。因此，体节衍生的F-腱蛋白是一种抑制信号，参与了NC细胞的节段性迁移模式及其在RS内的拓扑分离。
