Finlay J A, Day J R, Rittenhouse H G
Hybritech Incorporated, San Diego, California 92196-9006, USA.
Urology. 1999 Apr;53(4):746-51. doi: 10.1016/s0090-4295(98)00573-1.
The human tissue kallikrein family contains three closely related proteases: human kallikrein 1 (hK1), human kallikrein 2 (hK2), and prostate-specific antigen (PSA). The structural homology between these three proteins suggests potential cross-reactivity interference when different immunologic techniques are used. This study evaluated PSA and hK2 monoclonal antibody (mAb) and polyclonal antibody (pAb) reactivities to hK1, hK2, and PSA.
mAbs and pAbs to hK2 and PSA were evaluated using Western blot analysis on hK1, hK2, PSA, and seminal plasma.
pAbs to PSA and hK2 recognized all three human kallikreins, as well as fragments of hK2 and PSA. An mAb with minimal (less than 0.4%) cross-reactivity between PSA and hK2 and a cross-reactive mAb were found. mAbs specific to PSA or hK2 did not cross-react with the less homologous hK1 protein. A PSA mAb raised specifically to PSA fragments recognized both PSA and hK2 but did not cross-react with hK1. pAbs to hK1 cross-reacted slightly with PSA and not at all with hK2.
Both pAbs and mAbs to hK2 and PSA may exhibit immunocross-reactivity. pAbs to PSA or hK2 react with all three human tissue kallikreins. The potential for cross-reactivity should be considered in any clinical or research procedures that use hK1, hK2, and PSA antibodies.
人类组织激肽释放酶家族包含三种密切相关的蛋白酶：人类激肽释放酶1（hK1）、人类激肽释放酶2（hK2）和前列腺特异性抗原（PSA）。这三种蛋白质之间的结构同源性表明，当使用不同的免疫技术时，可能存在交叉反应干扰。本研究评估了PSA和hK2单克隆抗体（mAb）及多克隆抗体（pAb）对hK1、hK2和PSA的反应性。
使用蛋白质印迹分析评估针对hK2和PSA的mAb和pAb对hK1、hK2、PSA和精浆的反应性。
针对PSA和hK2的pAb识别所有三种人类激肽释放酶以及hK2和PSA的片段。发现一种在PSA和hK2之间交叉反应性极小（小于0.4%）的mAb和一种交叉反应性mAb。对PSA或hK2特异的mAb不与同源性较低的hK1蛋白发生交叉反应。一种专门针对PSA片段产生的PSA mAb识别PSA和hK2，但不与hK1发生交叉反应。针对hK1的pAb与PSA有轻微交叉反应，与hK2无交叉反应。
针对hK2和PSA的pAb和mAb均可能表现出免疫交叉反应性。针对PSA或hK2的pAb与所有三种人类组织激肽释放酶发生反应。在任何使用hK1、hK2和PSA抗体的临床或研究程序中，都应考虑交叉反应的可能性。
