Wood I S, Allison G G, Shirazi-Beechey S P
Department of Veterinary Preclinical Sciences, University of Liverpool, Brownlow Hill, L69 3BX, United Kingdom.
Biochem Biophys Res Commun. 1999 Apr 13;257(2):533-7. doi: 10.1006/bbrc.1999.0526.
d-Glucose and non-metabolisable analogues of D-glucose regulate the expression of intestinal SGLT1 at both transcriptional and post-transcriptional levels. In order to investigate the molecular mechanisms involved in the transcriptional regulation of the ovine intestinal SGLT1 gene, we have isolated an upstream element of about 1 kb in size. This DNA fragment contains a TATA box motif, 48 bp upstream of the transcriptional start site and includes transcription factor binding sites for HNF-1 and AP-2. We have shown that the ovine SGLT1 promoter fragment can drive the transcription of a reporter gene when transfected into the epithelial cell lines STC-1 and LLC-PK1, which endogenously express SGLT1. Deletion analyses of the promoter indicate that -66/+21 bp proximal sequence directs the highest level of reporter gene activity. There are one and possibly two sites of transcriptional suppression.
D-葡萄糖和D-葡萄糖的非代谢类似物在转录和转录后水平上调节肠道SGLT1的表达。为了研究参与绵羊肠道SGLT1基因转录调控的分子机制，我们分离出了一个大小约为1 kb的上游元件。该DNA片段包含一个TATA盒基序，位于转录起始位点上游48 bp处，并且包括HNF-1和AP-2的转录因子结合位点。我们已经表明，绵羊SGLT1启动子片段转染到内源性表达SGLT1的上皮细胞系STC-1和LLC-PK1中时，可以驱动报告基因的转录。启动子的缺失分析表明，-66 / + 21 bp近端序列指导报告基因活性的最高水平。存在一个以及可能两个转录抑制位点。
