Green V L, Speirs V, Landolt A M, Foy P M, Atkin S L
Department of Medicine, University of Hull, United Kingdom.
J Clin Endocrinol Metab. 1999 Apr;84(4):1340-5. doi: 10.1210/jcem.84.4.5619.
17Beta-hydroxysteroid dehydrogenase (17betaHSD) isoforms reversibly catalyze the final step in the formation of estradiol (E2) from estrone (E1) and the formation of testosterone from androstenedione. We have investigated 17betaHSD type 1, 2, 3, and 4 gene expression and 17betaHSD estrogenic activity in human anterior pituitary adenomas. 17BetaHSD messenger ribonucleic acid (mRNA) expression was studied by RT-PCR in 42 pituitary tumors and 3 normal pituitaries, 17betaHSD activity was studied in 11 tumors and 17betaHSD type 1 was immunolocalized in vitro in 6 tumors. 17BetaHSD type 1 gene expression was detected in 34 of 42 adenomas in all tumor subtypes; 17betaHSD type 2 mRNA was detected in 18 of 42 adenomas, but not in prolactinomas; 17betaHSD type 3 mRNA was detected in 12 of 42 adenomas, but not in corticotropinomas; 17betaHSD type 4 was expressed in 20 of 42 adenomas by all adenoma subtypes. Reversible 17betaHSD activity was found in 9 of 11 adenomas, and 17betaHSD type 1 immunopositivity was cytoplasmically distributed in all 6 adenomas in vitro. All 4 17betaHSD isoforms are variably expressed in human anterior pituitary adenomas, which also show 17betaHSD enzyme activity, suggesting that 17betaHSD may play an important role in regulating the local cellular levels of estradiol.
17β-羟基类固醇脱氢酶（17βHSD）同工型可逆地催化雌酮（E1）形成雌二醇（E2）以及雄烯二酮形成睾酮的最后一步反应。我们研究了人垂体前叶腺瘤中17βHSD 1型、2型、3型和4型的基因表达以及17βHSD的雌激素活性。通过逆转录聚合酶链反应（RT-PCR）研究了42例垂体肿瘤和3例正常垂体中17βHSD信使核糖核酸（mRNA）的表达，在11例肿瘤中研究了17βHSD的活性，并在体外对6例肿瘤进行了17βHSD 1型的免疫定位。在所有肿瘤亚型的42例腺瘤中，有34例检测到17βHSD 1型基因表达；42例腺瘤中有18例检测到17βHSD 2型mRNA，但在催乳素瘤中未检测到；42例腺瘤中有12例检测到17βHSD 3型mRNA，但在促肾上腺皮质激素瘤中未检测到；42例腺瘤中有20例通过所有腺瘤亚型表达了17βHSD 4型。在11例腺瘤中的9例中发现了可逆的17βHSD活性，并且在体外所有6例腺瘤中17βHSD 1型免疫阳性均呈细胞质分布。所有4种17βHSD同工型在人垂体前叶腺瘤中均有不同程度的表达，这些腺瘤也表现出17βHSD酶活性，提示17βHSD可能在调节雌二醇的局部细胞水平中起重要作用。
