Zhang J, Shi Q, Pan S, Zhang X, Shan XS, Yu L
Department of Biology, Nanjing Normal University, Nanjing, 210097, P. R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 1999 Apr 10;16(2):103-107.
To determine the influence of aging and sex on the segregation of chromosome 21. METHODS: Lymphocytes were obtained from healthy donors of different ages, both sexes, and were treated with cytochalasin B. Binucleated cells were hybridized with chromosome 21 specific probe, and simultaneously, chromosome 21 loss and non-disjunction were detected. RESULTS: The coefficients of correlation between age and binucleated cells containing 4, 2 and 6 signals were -0.35(P<0.01), 0.18 and 0.38(P<0.01) respectively. The coefficients of correlation between age and nondisjunction of chromosome 21 and micronuclei were 0.56(P<0.01) and 0.70(P<0.01) respectively. CONCLUSION: There is a significant increase with age in the number of micronuclei and missegregation. Nondisjunction of chromosome 21 is much more frequent than loss in vivo and in vitro. No significant difference in nondisjunctioin between male and female was noted. Age effect is more significant in female than in male.
确定衰老和性别对21号染色体分离的影响。方法：从不同年龄、性别的健康供体获取淋巴细胞，并用细胞松弛素B处理。双核细胞与21号染色体特异性探针杂交，同时检测21号染色体丢失和不分离情况。结果：年龄与含4、2和6个信号的双核细胞之间的相关系数分别为-0.35（P<0.01）、0.18和0.38（P<0.01）。年龄与21号染色体不分离及微核之间的相关系数分别为0.56（P<0.01）和0.70（P<0.01）。结论：微核数量和错误分离随年龄显著增加。21号染色体不分离在体内和体外比丢失更频繁。未观察到男性和女性在不分离方面的显著差异。年龄对女性的影响比男性更显著。
